Presto Elgstoen K B, Jellum E
Institute of Clinical Biochemistry, Rikshospitalet, Oslo, Norway.
Electrophoresis. 1997 Sep;18(10):1857-60. doi: 10.1002/elps.1150181022.
Capillary electrophoresis (CE) equipped with a diode-array detector have been used to determine diagnostic metabolites occurring in urine of patients with various diseases. The urine samples were injected directly onto the CE instrument without any pretreatment. Identification of abnormal metabolites was based on relative migration times and characteristic diode-array spectra. The CE-method readily diagnosed alkaptonuria, neuroblastoma and liver failure due to galactosemia. The simple and automated CE method appears to be suitable for implementation as a screening test in analytical systems aimed at diagnosis of metabolic disorders.
配备二极管阵列检测器的毛细管电泳（CE）已用于测定患有各种疾病的患者尿液中出现的诊断性代谢物。尿液样本未经任何预处理直接注入CE仪器。异常代谢物的鉴定基于相对迁移时间和特征性二极管阵列光谱。CE方法能够轻松诊断出尿黑酸尿症、神经母细胞瘤和半乳糖血症所致的肝衰竭。这种简单且自动化的CE方法似乎适合作为一种筛查测试应用于旨在诊断代谢紊乱的分析系统中。
