Grannis G F
Am J Clin Pathol. 1976 Jul;66(1):206-22.
Enzyme survey specimens were prepared by spiking portions of a normal serum pool with creatinine, urea, and five enzymes (lactate dehydrogenase, creatine phosphokinase, alkaline phosphatase, and aspartate and alanine aminotransferases), and preparing admixtures of the spiked pools with the original serum. This admixture technic established linear interspecimen relationships that could be confirmed by analyses for creatinine and urea nitrogen. Both ethylene glycol-stabilized liquid serum specimens and lyophilized specimens were prepared as sets of six to eight samples having six concentrations of each enzyme. The sets were distributed on five occasions to about ten laboratories that were widely separated geographically, and the specimens were analyzed by a variety of methods, by various instrumental systems, and in different reaction conditions, and results were reported in diverse units. This report describes how the analytic data obtained through the use of these specimens that were specifically designed for survey purposes can be analyzed statistically to provide meaningful assessments of laboratory performance in enzyme analyses.
酶检测标本的制备方法是，在正常血清混合液的部分样本中加入肌酐、尿素和五种酶（乳酸脱氢酶、肌酸磷酸激酶、碱性磷酸酶、天冬氨酸氨基转移酶和丙氨酸氨基转移酶），然后将加样后的混合液与原始血清制备成混合物。这种混合技术建立了标本间的线性关系，这可以通过肌酐和尿素氮分析得到证实。乙二醇稳定化液体血清标本和冻干标本均制备成每组六到八个样本，每种酶有六个浓度。这些样本分五次分发给大约十个地理位置分散的实验室，样本通过多种方法、各种仪器系统并在不同反应条件下进行分析，结果以不同单位报告。本报告描述了如何对通过使用这些专门为检测目的设计的标本获得的分析数据进行统计分析，以对酶分析中的实验室性能提供有意义的评估。
