Di Perri G, Olliaro P, Nardi S, Allegranzi B, Deganello R, Vento S, Lanzafame M, Cazzadori A, Bonora S, Concia E
Institute of Immunology and Infectious Diseases, University of Verona, Italy.
Trans R Soc Trop Med Hyg. 1997 Jul-Aug;91(4):403-5. doi: 10.1016/s0035-9203(97)90257-1.
Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3 x 5 min and 3 x 20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF. Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts < or = 100/microL). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts > 100/microL and 0.458 and 0.966 respectively for counts < or = 100/microL. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P < 0.001) but less specific (P = 0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.
在对32名布隆迪患者进行抗疟治疗及随访期间，比较了外周血中恶性疟原虫感染的三种检测方法：试纸条抗原捕获检测法、标准厚血膜法（TBF）和延长厚血膜检查法（PTBF）（分别为3次每次5分钟和3次每次20分钟检查）。每天通过与全白细胞计数（由库尔特计数器测定）比较来确定疟原虫血症，直到连续2天PTBF检测未发现疟原虫。每种检测方法累计进行了231次观察：PTBF检测64次为阴性，167次为阳性（59次疟原虫计数≤100/微升）。与PTBF相比，对于疟原虫计数＞100/微升，TBF和试纸条检测法的敏感性均为1.0；对于计数≤100/微升，敏感性分别为0.458和0.966。总体而言，试纸条检测法比TBF显著更敏感（0.988对0.808；P＜0.001）但特异性更低（P = 0.013）。试纸条检测法在监测药物治疗反应和检测低疟原虫血症方面具有潜在用途。
