Cetinkale O, Sengul R, Bilgic L, Bolayirli M, Senel O, Burcak G
Department of Plastic & Reconstructive Surgery, Cerrahpasa Medical Faculty, Istanbul University, Turkey.
Ann Plast Surg. 1997 Nov;39(5):505-15. doi: 10.1097/00000637-199711000-00011.
The purpose of this study was to investigate the role of neutrophils in ischemic tissue injury and the possible inhibition by pretreatment with FK506, a neutrophilic modulating agent. A dorsal caudally based skin flap (3 x 9 cm) was used as an ischemic injury model in experimental groups. Prior to flap elevation, FK506 at doses of 0.3 mg per kilogram (group 2), 0.5 mg per kilogram (group 3), and 1.0 mg per kilogram (group 4) was given for 3 days intramuscularly. The relationship among neutrophil accumulation (histopathologically), myeloperoxidase (MPO) activity, malondialdehyde (MDA) content (biochemically) of the flap tissue, and flap survival were studied. Skin flaps showed reduced necrosis in the FK506-treated groups (p < 0.08, p < 0.0001, and p < 0.0001 respectively). The increase in accumulation of neutrophils, and MDA and MPO levels (which were induced by ischemia) observed 1 and 24 hours after flap elevation was diminished by FK506 pretreatment. The increased neutrophilic infiltration, and raised tissue MDA content and MPO activity revealed involvement of both free radical production and neutrophils in ischemia. This injury was decreased by FK506, probably by inhibition of neutrophilic chemotaxis, infiltration, and releasing factors.
本研究的目的是探讨中性粒细胞在缺血性组织损伤中的作用，以及中性粒细胞调节剂FK506预处理可能产生的抑制作用。在实验组中，采用背尾侧皮肤皮瓣（3×9厘米）作为缺血性损伤模型。在皮瓣掀起前，分别以每千克0.3毫克（第2组）、0.5毫克（第3组）和1.0毫克（第4组）的剂量肌肉注射FK506，持续3天。研究了皮瓣组织中中性粒细胞聚集（组织病理学）、髓过氧化物酶（MPO）活性、丙二醛（MDA）含量（生化指标）与皮瓣存活之间的关系。在FK506治疗组中，皮瓣坏死减少（分别为p<0.08、p<0.0001和p<0.0001）。FK506预处理减少了皮瓣掀起后1小时和24小时观察到的中性粒细胞聚集增加以及MDA和MPO水平升高（由缺血诱导）。中性粒细胞浸润增加、组织MDA含量升高和MPO活性升高表明自由基产生和中性粒细胞均参与了缺血过程。FK506可能通过抑制中性粒细胞趋化、浸润和释放因子而减轻这种损伤。
