Plautz J D, Kaneko M, Hall J C, Kay S A
Department of Cell Biology and National Science Foundation Center for Biological Timing, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Science. 1997 Nov 28;278(5343):1632-5. doi: 10.1126/science.278.5343.1632.
Transgenic Drosophila that expressed either luciferase or green fluorescent protein driven from the promoter of the clock gene period were used to monitor the circadian clock in explanted head, thorax, and abdominal tissues. The tissues (including sensory bristles in the leg and wing) showed rhythmic bioluminescence, and the rhythms could be reset by light. The photoreceptive properties of the explanted tissues indicate that unidentified photoreceptors are likely to contribute to photic signal transduction to the clock. These results show that autonomous circadian oscillators are present throughout the body, and they suggest that individual cells in Drosophila are capable of supporting their own independent clocks.
利用从生物钟基因period的启动子驱动表达荧光素酶或绿色荧光蛋白的转基因果蝇，来监测离体头部、胸部和腹部组织中的昼夜节律钟。这些组织（包括腿部和翅膀上的感觉刚毛）呈现出节律性生物发光，并且这些节律能够被光重置。离体组织的感光特性表明，未明确的光感受器可能有助于光信号向生物钟的转导。这些结果表明，自主的昼夜节律振荡器遍布全身，并且表明果蝇中的单个细胞能够维持其自身独立的生物钟。
