Khimani A H, Lim M, Graf T G, Smith T F, Ruprecht R M
Laboratory of Viral Pathogenesis, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Virology. 1997 Nov 10;238(1):64-7. doi: 10.1006/viro.1997.8814.
We report the complete nucleotide sequence of the genome of Rauscher murine leukemia virus (R-MuLV), the replication-competent helper virus present in the Rauscher virus complex, and its phylogenetic relationship with other murine leukemia virus genomes. An overall sequence identity of 97.6% was found between R-MuLV and the Friend helper virus (F-MuLV), and the two viruses were closely related on the phylogenetic trees constructed from either gag, pol, or env sequences. Moloney murine leukemia virus (Mo-MuLV) was the next closest relative to R-MuLV and F-MuLV on all trees, followed by Akv and radiation leukemia virus (RadLV). The most distantly related helper virus was Hortulanus murine leukemia virus (Ho-MuLV). Interestingly, Cas-Br-E branched with Mo-MuLV on the gag and pol trees, whereas on the env tree, it revealed the highest degree of relatedness to Ho-MuLV, possibly due to an ancient recombination with an Ho-MuLV ancestor. In summary, a phylogenetic analysis involving various MuLVs has been performed, in which the postulated close relationship between R-MuLV and F-MuLV has been confirmed, consistent with the pathobiology of the two viruses.
我们报告了劳舍尔鼠白血病病毒（R-MuLV）基因组的完整核苷酸序列，它是劳舍尔病毒复合物中具有复制能力的辅助病毒，以及它与其他鼠白血病病毒基因组的系统发育关系。在R-MuLV和弗瑞德辅助病毒（F-MuLV）之间发现总体序列同一性为97.6%，并且在根据gag、pol或env序列构建的系统发育树上，这两种病毒密切相关。莫洛尼鼠白血病病毒（Mo-MuLV）在所有树上是仅次于R-MuLV和F-MuLV的近亲，其次是Akv和辐射白血病病毒（RadLV）。亲缘关系最远的辅助病毒是霍图拉努斯鼠白血病病毒（Ho-MuLV）。有趣的是，Cas-Br-E在gag和pol树上与Mo-MuLV分支在一起，而在env树上，它显示出与Ho-MuLV的最高相关度，这可能是由于与Ho-MuLV祖先的一次古老重组。总之，已经对各种鼠白血病病毒进行了系统发育分析，其中R-MuLV和F-MuLV之间假定的密切关系得到了证实，这与这两种病毒的病理生物学一致。
