McGuire T C, Zhang W, Hines M T, Henney P J, Byrne K M
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
Virology. 1997 Nov 10;238(1):85-93. doi: 10.1006/viro.1997.8795.
Horses with equine infectious anemia virus (EIAV) have episodes of viremia and disease; however, most eventually become inapparent carriers. A possible mechanism of control is cytotoxic T lymphocytes (CTL). To evaluate CTL in inapparent carriers with low viral loads, peripheral blood mononuclear cells (PBMC) were stimulated in vitro with autologous EIAV-infected PBMC and human IL-2 to detect memory CTL (CTLm). In initial studies, three carriers had CTLm and one of these had low-level effector CTL (CTLe). The CTLm were restricted by equine lymphocyte alloantigen-A (ELA-A) locus encoded MHC class I molecules on autologous equine kidney (EK) target cells. In addition, EK cells did not express MHC class II molecules. The CTLm frequency in PBMC from five inapparent carriers infected for 22 to 50 months was determined by limiting dilution analysis. PBMC were diluted, stimulated, and tested on EK cell targets infected with EIAV and recombinant vaccinia viruses expressing EIAV Env or Gag/Pr proteins. All five carriers had CTLm to EIAV-infected targets, while four had CTLm to targets expressing Env and four had CTLm to targets expressing Gag/Pr proteins. The CTLm frequency range was 60 to 468 per 10(6) PBMC to EIAV-infected targets, 4 to 286 to Env-expressing targets, and 25 to 190 to Gag/Pr-expressing targets. These results should facilitate the identification of epitopes recognized by predominant CTLm from horses controlling a lentivirus infection.
感染马传染性贫血病毒（EIAV）的马匹会出现病毒血症和疾病发作；然而，大多数最终会成为隐性携带者。一种可能的控制机制是细胞毒性T淋巴细胞（CTL）。为了评估低病毒载量隐性携带者中的CTL，外周血单个核细胞（PBMC）在体外与自体感染EIAV的PBMC和人白细胞介素-2一起刺激，以检测记忆CTL（CTLm）。在初步研究中，三名携带者有CTLm，其中一人有低水平效应CTL（CTLe）。CTLm受马淋巴细胞同种异体抗原-A（ELA-A）位点编码的MHC I类分子限制，作用于自体马肾（EK）靶细胞。此外，EK细胞不表达MHC II类分子。通过有限稀释分析确定了5名感染22至50个月的隐性携带者PBMC中的CTLm频率。PBMC被稀释、刺激，并在感染EIAV以及表达EIAV Env或Gag/Pr蛋白的重组痘苗病毒感染的EK细胞靶标上进行检测。所有五名携带者对感染EIAV的靶标都有CTLm，而四名对表达Env的靶标有CTLm，四名对表达Gag/Pr蛋白的靶标有CTLm。CTLm频率范围为每10(6) PBMC中60至468个针对感染EIAV的靶标、4至286个针对表达Env的靶标以及25至190个针对表达Gag/Pr蛋白的靶标。这些结果应有助于鉴定控制慢病毒感染的马匹中主要CTLm识别的表位。
