Russell K E, Walker K M, Miller R T, Sellon D C
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Am J Vet Res. 1998 Aug;59(8):1009-15.
To determine blood protein concentration, immunoglobulin concentration, and lymphocyte profiles in equine infectious anemia virus (EIAV) seropositive, naturally infected horses without clinical signs of disease.
26 clinically normal seropositive horses, 6 febrile ponies with experimentally induced EIA, and 52 clinically normal seronegative horses and ponies.
Serum and EDTA-anticoagulated blood were obtained from all horses and ponies, and total serum protein and albumin concentrations, immunoglobulin concentrations, and blood lymphocyte subset counts were determined.
Compared with seronegative horses, EIAV seropositive inapparent carrier horses had no significant difference in serum reverse transcriptase activity, PCV, or platelet count. Inapparent carrier horses had increased plasma total solids and serum globulin concentrations and decreased serum albumin concentration and albumin-to-globulin ratio. Total serum immunoglobulin and serum IgM concentrations were increased. Inapparent carrier horses had significantly decreased percentages of CD5+ and CD4+ blood lymphocytes.
Serum protein and lymphocyte subset changes in EIAV-infected inapparent carrier horses are consistent with immune activation or chronic inflammation, both of which may, in part, be the result of virus-induced polyclonal B-cell activation.
EIAV seropositive horses have immune-related abnormalities consistent with ongoing viral activity regardless of the duration they have been infected, even when the usual signs of disease (anemia, fever, weight loss) are not apparent.
测定马传染性贫血病毒（EIAV）血清学阳性、自然感染且无疾病临床症状的马匹的血液蛋白浓度、免疫球蛋白浓度和淋巴细胞谱。
26匹临床正常的血清学阳性马匹、6匹实验性诱导感染EIA的发热小马，以及52匹临床正常的血清学阴性马匹和小马。
采集所有马匹和小马的血清及乙二胺四乙酸（EDTA）抗凝血液，测定血清总蛋白和白蛋白浓度、免疫球蛋白浓度以及血液淋巴细胞亚群计数。
与血清学阴性马匹相比，EIAV血清学阳性的隐性带毒马匹在血清逆转录酶活性、红细胞压积或血小板计数方面无显著差异。隐性带毒马匹的血浆总固体和血清球蛋白浓度升高，血清白蛋白浓度和白蛋白与球蛋白比值降低。血清总免疫球蛋白和血清IgM浓度升高。隐性带毒马匹的CD5⁺和CD4⁺血液淋巴细胞百分比显著降低。
EIAV感染的隐性带毒马匹的血清蛋白和淋巴细胞亚群变化与免疫激活或慢性炎症一致，这两者可能部分是病毒诱导的多克隆B细胞激活的结果。
EIAV血清学阳性马匹存在与持续病毒活动相关的免疫异常，无论其感染持续时间长短，即使没有疾病的常见症状（贫血、发热、体重减轻）。
