Miyahara T, Shibamoto T, Wang H G, Koyama S
Department of Physiology, Shinshu University School of Medicine, Matsumoto, Japan.
J Appl Physiol (1985). 1997 Nov;83(5):1508-16. doi: 10.1152/jappl.1997.83.5.1508.
We determined the roles of circulating blood components and chemical mediators in anaphylactic vasoconstriction and microvascular permeability during Ascaris suum antigen-induced anaphylaxis in isolated canine lungs. Either the right or left lower lobe served as the control lung, which was perfused with autologous blood, and the contralateral lobe from the same dog was examined for the effect of albumin Krebs-Henseleit solution (Krebs) or of blockers of various vasoconstrictors with blood perfusion. Pulmonary vasoconstriction occurred after injection of the antigen (15 mg) in both the blood- and Krebs-perfused lungs. However, the percent change of peak pulmonary vascular resistance in the Krebs-perfused lungs tended to be greater than that in the blood-perfused lungs (689.9 +/- 289.3 and 389.3 +/- 171.9%, respectively). This increased peak pulmonary vascular resistance was attenuated similarly by pretreatment with indomethacin (1.1 x 10(-4) M; cyclooxygenase inhibitor), AA-2414 (10(-5) M; thromboxane-receptor antagonist), or a combination of TCV-309 (10(-5) M; platelet-activating factor-receptor antagonist), diphenhydramine (1.7 x 10(-4) M, histamine H1-receptor antagonist), and indomethacin but not by pretreatment with TCV-309 or diphenhydramine alone. The filtration coefficient, an index of vascular permeability, did not change significantly at 15 or 60 min after the antigen in all groups. These findings suggest that anaphylactic vasoconstriction in the isolated canine lung is independent of circulating blood components. Thromboxane is the major mediator for the anaphylactic vasoconstriction. Anaphylaxis does not increase pulmonary vascular permeability in isolated canine lungs.
我们确定了循环血液成分和化学介质在猪蛔虫抗原诱导的离体犬肺过敏反应中过敏血管收缩和微血管通透性方面的作用。左右下叶中的一叶作为对照肺，用自体血液灌注，而来自同一只狗的对侧肺叶则用于检测白蛋白克氏-亨氏液（Krebs液）或各种血管收缩剂阻滞剂对血液灌注的影响。在血液灌注肺和Krebs液灌注肺中注射抗原（15毫克）后均出现肺血管收缩。然而，Krebs液灌注肺中肺血管阻力峰值的变化百分比往往大于血液灌注肺（分别为689.9±289.3%和389.3±171.9%）。预先用吲哚美辛（1.1×10⁻⁴M；环氧化酶抑制剂）、AA-2414（10⁻⁵M；血栓素受体拮抗剂）或TCV-309（10⁻⁵M；血小板活化因子受体拮抗剂）、苯海拉明（1.7×10⁻⁴M，组胺H1受体拮抗剂）和吲哚美辛联合预处理可类似地减弱这种肺血管阻力峰值的增加，但单独用TCV-309或苯海拉明预处理则无此作用。血管通透性指标滤过系数在所有组中抗原注射后15分钟或60分钟时均无显著变化。这些发现表明，离体犬肺中的过敏血管收缩与循环血液成分无关。血栓素是过敏血管收缩的主要介质。过敏反应不会增加离体犬肺的肺血管通透性。
