Schaap F G, Hamers L, Van der Vusse G J, Glatz J F
Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, The Netherlands.
Biochim Biophys Acta. 1997 Oct 9;1354(1):29-34. doi: 10.1016/s0167-4781(97)00121-8.
Mitochondrial oxidation of long-chain fatty acids provides the majority of the energy required for cardiac functioning. Several proteins, including the integral membrane protein FATP (Fatty Acid-Transport Protein), are being implicated in the process of myocardial fatty acid uptake. To further characterize the role of FATP in rat myocardial fatty acid utilization, cDNA encoding rat FATP was cloned. The inferred amino acid sequence indicates that rat FATP is highly homologous (97%) with its murine equivalent. Moreover, rodent FATPs share several well-conserved regions with putative counterparts found in yeast and nematode. Given the large evolutionary distance between these species, these regions might be important for protein function. The predicted membrane topology of rat FATP is discussed.
长链脂肪酸的线粒体氧化作用为心脏功能提供了大部分所需能量。包括整合膜蛋白脂肪酸转运蛋白（FATP）在内的几种蛋白质与心肌脂肪酸摄取过程有关。为了进一步阐明FATP在大鼠心肌脂肪酸利用中的作用，克隆了编码大鼠FATP的cDNA。推导的氨基酸序列表明，大鼠FATP与其小鼠对应物高度同源（97%）。此外，啮齿动物的FATP与酵母和线虫中发现的假定对应物有几个保守区域。鉴于这些物种之间进化距离较大，这些区域可能对蛋白质功能很重要。本文讨论了大鼠FATP预测的膜拓扑结构。
