Schaffer J E, Lodish H F
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Cell. 1994 Nov 4;79(3):427-36. doi: 10.1016/0092-8674(94)90252-6.
Long chain fatty acids (LCFAs) are an important energy substrate used by cardiac myocytes and other cells, but the mechanism whereby these molecules cross the plasma membrane is poorly understood. We used an expression cloning strategy and a cDNA library from 3T3-L1 adipocytes to identify a cDNA that, when expressed in cultured cells, augments uptake of LCFAs. This cDNA encodes a novel 646 amino acid fatty acid transport protein (FATP) with six predicted membrane-spanning regions and that is integrally associated with membranes. Immunocytochemistry and subcellular fractionation of 3T3-L1 adipocytes show that FATP is localized to the plasma membrane. We propose that FATP is a plasma membrane transporter for LCFAs.
长链脂肪酸（LCFAs）是心肌细胞和其他细胞所利用的一种重要能量底物，但这些分子穿过质膜的机制却鲜为人知。我们采用表达克隆策略，并利用来自3T3-L1脂肪细胞的cDNA文库来鉴定一种cDNA，当它在培养细胞中表达时，能增强长链脂肪酸的摄取。该cDNA编码一种新的含646个氨基酸的脂肪酸转运蛋白（FATP），它有6个预测的跨膜区域，且与膜紧密相连。对3T3-L1脂肪细胞进行免疫细胞化学和亚细胞分级分离显示，FATP定位于质膜。我们推测FATP是长链脂肪酸的质膜转运体。
