Shi Y, Wu Y, Jin F, Yan F, Yu X, Yuan Q
Department of Food Engineering, Dalian Institute of Light Industry, China.
Chin J Biotechnol. 1997;13(1):59-62.
In order to achieve high activity of enzymatic catalysis, the method of permealizing and crosslinking treatment has been suggested for immobilizing cells with endocellular enzymes. Polyethene-polyamine and glutardehyde were used to enhance the activity and stability of E. coli having aspartase entrapped in k-carrageenan gel and intact P. dacunhae cells having aspartate-beta-decarbonoxylase. The strength and enlargement effects, which favor the performance of catalysis in a packed bed reactor and a membrane reactor, respectively, were also obtained from the experiment results.
为了实现酶催化的高活性，已提出采用渗透和交联处理的方法来固定含有胞内酶的细胞。使用聚乙烯多胺和戊二醛来提高包埋在κ-卡拉胶凝胶中的含天冬氨酸酶的大肠杆菌以及完整的含有天冬氨酸β-脱羧酶的达库尼亚假单胞菌细胞的活性和稳定性。实验结果还分别获得了有利于在填充床反应器和膜反应器中进行催化性能的强度和放大效应。
