[The effect of 1,4-benzodiazepine derivatives on 35S-tert-butylbicyclophosphorothionate binding in the brain of inbred mice with differing emotional stress reactions].

The effect of alprazolam, cinazepam, and hydazepam on the binding of 35S-tert-butylbicyclophosphorothionate with the brain membranes of inbred Balb/c and C57B1/6 mice with a different type of emotional-stress reaction was studied. The displacement curve of 35S-TBPS bound with alprazolam and cinazepam was two-phase in character with different degree of inhibition in the nanomolar and micromolar areas. Hydazepam displaced the bound radioligand only in micromolar concentrations. Displacement of the bound radioligand in the brain membranes began with lower concentrations of the used benzodiazepins in Balb/c animals than in C57B1/6 animals. The emotional-stress effect led to shift of the displacement curves of the bound 35S-TBPS in the direction of higher acting concentrations of the benzodiazepins in the brain membranes of mice of both lines.