胎儿生长激素、胰岛素样生长因子I、生长激素结合蛋白及胰岛素样生长因子结合蛋白-3的发育模式

Developmental pattern of fetal growth hormone, insulin-like growth factor I, growth hormone binding protein and insulin-like growth factor binding protein-3.

作者信息

Pirazzoli P, Cacciari E, De Iasio R, Pittalis M C, Dallacasa P, Zucchini S, Gualandi S, Salardi S, David C, Boschi S

机构信息

Department of Paediatrics, St Orsola Hospital, University of Bologna, Italy.

出版信息

Arch Dis Child Fetal Neonatal Ed. 1997 Sep;77(2):F100-4. doi: 10.1136/fn.77.2.f100.

DOI:10.1136/fn.77.2.f100

PMID:9377129

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1720686/

Abstract

AIMS

To evaluate the developmental pattern of fetal growth hormone (GH), insulin-like growth factor I (IGF-I), GH binding protein (GHBP) and IGF binding protein-3 (IGF-3); to determine the implications for fetal growth.

METHODS

Serum GH, IGF-I, GHBP and IGFBP-3 were measured in 53 fetuses, 41 aged 20-26 weeks (group A) and 12 aged 31-38 weeks (group B). Fetal blood samples were obtained by direct puncture of the umbilical vein in utero. Fetal blood samples were taken to rule out beta thalassaemia, chromosome alterations, mother to fetus transmissible infections, and for maternal rhesus factor. GHBP was determined by gel filtration chromatography of serum incubated overnight with 125I-GH. GH, IGF-I and IGFBP-3 were determined by radioimmunoassay.

RESULTS

Fetal serum GH concentrations in group A (median 29 micrograms/l, range 11-92) were significantly higher (P < 0.01) than those of group B (median 16.7 micrograms/l, range 4.5-29). IGF-I in group A (median 20 micrograms/l, range 4.1-53.3) was significantly lower (P < 0.01) than in group B (median 75.2 micrograms/l, range 27.8-122.3). Similarly, IGFBP-3 concentrations in group A (median 950 micrograms/l, range 580-1260) were significantly lower than those of group B (median 1920 micrograms/l, range 1070-1770). There was no significant difference between GHBP values in group A (median 8.6%, range 6.6-12.6) and group B (median 8.3%, range 6-14.3). Gestational age correlated positively with IGF-I concentrations (P < 0.0001) and IGFBP-3 (P < 0.0001) and negatively with GH (P < 0.0001). GHBP values did not correlate with gestational age. Multiple regression analysis showed a negative correlation between GH:IGF-I ratio and fetal growth indices

CONCLUSIONS

The simultaneous evaluation of fetal GH, IGF-I, IGFBP-3 and GHBP suggests that the GH-IGF-I axis might already be functional in utero. The progressive improvement in the efficiency of this axis in the last part of gestation does not seem to be due to an increase in GH receptors.

摘要

目的

评估胎儿生长激素（GH）、胰岛素样生长因子I（IGF-I）、生长激素结合蛋白（GHBP）和胰岛素样生长因子结合蛋白-3（IGFBP-3）的发育模式；确定其对胎儿生长的影响。

方法

对53例胎儿进行血清GH、IGF-I、GHBP和IGFBP-3检测，其中41例年龄为20 - 26周（A组），12例年龄为31 - 38周（B组）。通过子宫内直接穿刺脐静脉获取胎儿血样。采集胎儿血样以排除β地中海贫血、染色体改变、母婴传播感染，并检测母亲的恒河猴因子。通过将血清与125I - GH孵育过夜后进行凝胶过滤色谱法测定GHBP。通过放射免疫分析法测定GH、IGF-I和IGFBP-3。

结果

A组胎儿血清GH浓度中位数为29微克/升，范围为11 - 92微克/升，显著高于B组（中位数16.7微克/升，范围4.5 - 29微克/升，P < 0.01）。A组IGF-I中位数为20微克/升，范围为4.1 - 53.3微克/升，显著低于B组（中位数75.2微克/升，范围27.8 - 122.3微克/升，P < 0.01）。同样，A组IGFBP-3浓度中位数为950微克/升，范围为580 - 1260微克/升，显著低于B组（中位数1920微克/升，范围1070 - 1770微克/升）。A组GHBP值中位数为8.6%，范围为6.6 - 12.6%，与B组（中位数8.3%，范围6 - 14.3%）无显著差异。孕周与IGF-I浓度（P < 0.0001）和IGFBP-3（P < 0.0001）呈正相关，与GH呈负相关（P < 0.0001）。GHBP值与孕周无相关性。多元回归分析显示GH:IGF-I比值与胎儿生长指标呈负相关。