Parr E J, Myles E B, Hanani M, Seror D, Riabowol K T, Sharkey K A
Neuroscience Research Group, Department of Physiology and Biophysics, The University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1.
Cell Tissue Res. 1997 Oct;290(1):21-9. doi: 10.1007/s004410050903.
The Fas ligand induces apoptosis in activated immunocytes that express the Fas receptor. Fas-ligand transcripts have been found previously in murine intestine but the intestinal tissues that express Fas ligand have not been identified. We used immunohistochemistry to examine the expression of the Fas ligand in the enteric nervous system of rats, mice, guinea-pigs, ferrets and humans. Fas-ligand immunoreactivity was detectable in enteric nerve fibres and neurons in all species tested, representing 25%-50% of the neurons in rats, mice and guinea-pigs. An antigen of approximately 48 kDa was detected by Western blot analysis with Fas-ligand antiserum in the dissected enteric plexuses of duodenum from a C3H/HeJ mouse. In gld mice that harbour a Fas-ligand mutation, Fas-ligand immunoreactivity was slightly more intense in neurons and fibres and was also apparent in submucosal lymphocytes. In the myenteric plexuses of guinea-pig ileum and human colon, Fas-ligand immunoreactivity was not contained in neurons exhibiting nicotinamide-adenine dinucleotide phosphate-diaphorase activity. In the submucosal plexus of guinea-pig ileum, labelled neurons included some neuropeptide-Y-containing neurons but none with vasoactive intestinal polypeptide. We conclude that the Fas ligand is expressed by a large subset of enteric neurons and may provide the basis for cytotoxic neuroimmune interactions in the intestines.
Fas配体可诱导表达Fas受体的活化免疫细胞发生凋亡。此前已在小鼠肠道中发现Fas配体转录本，但尚未鉴定出表达Fas配体的肠道组织。我们采用免疫组织化学方法检测大鼠、小鼠、豚鼠、雪貂和人类肠神经系统中Fas配体的表达。在所有受试物种的肠神经纤维和神经元中均检测到Fas配体免疫反应性，在大鼠、小鼠和豚鼠中，这占神经元的25%-50%。用Fas配体抗血清进行蛋白质免疫印迹分析，在一只C3H/HeJ小鼠十二指肠的分离肠丛中检测到一种约48 kDa的抗原。在携带Fas配体突变的gld小鼠中，Fas配体免疫反应性在神经元和纤维中略强，在黏膜下淋巴细胞中也很明显。在豚鼠回肠和人类结肠的肌间神经丛中，显示烟酰胺腺嘌呤二核苷酸磷酸黄递酶活性的神经元中不含有Fas配体免疫反应性。在豚鼠回肠的黏膜下神经丛中，标记的神经元包括一些含神经肽Y的神经元，但不含血管活性肠肽的神经元。我们得出结论，Fas配体由大部分肠神经元表达，可能为肠道中的细胞毒性神经免疫相互作用提供基础。
