Toole L, Belai A, Burnstock G
Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK.
Cell Tissue Res. 1998 Mar;291(3):385-94. doi: 10.1007/s004410051008.
The neurochemical composition of nerve fibres and cell bodies in the myenteric plexus of the proventriculus, stomach and small and large intestines of the golden hamster was investigated by using immunohistochemical and histochemical techniques. In addition, the procedures for localising nitric-oxide-utilising neurones by histochemical (NADPH-diaphorase) and immunohistochemical (nitric oxide synthase) methods were compared. The co-localisation of vasoactive intestinal polypeptide and nitric oxide synthase in the myenteric plexus of all regions of the gut was also assessed. The results demonstrated the presence of nerve fibres and nerve cell bodies immunoreactive to protein gene product, vasoactive intestinal polypeptide, substance P, calcitonin gene-related peptide, tyrosine hydroxylase, 5-hydroxytryptamine and nitric oxide synthase in all regions of the gastrointestinal tract examined. The pattern of distribution of immunoreactive nerve fibres and nerve cell bodies containing the above markers was found to vary in different regions of the gut. Myenteric neurones and nerve fibres containing immunoreactivity to nitric oxide synthase and NADPH-diaphorase reactivity, however, were shown to have an identical distribution throughout the gut. In contrast to some studies on the guinea-pig and rat, the co-existence of vasoactive intestinal polypeptide and nitric oxide synthase was seen in only a small population of myenteric neurones.
采用免疫组织化学和组织化学技术，对金黄仓鼠前胃、胃、小肠和大肠肌间神经丛中神经纤维和细胞体的神经化学成分进行了研究。此外，还比较了用组织化学方法（还原型辅酶Ⅱ黄递酶）和免疫组织化学方法（一氧化氮合酶）定位利用一氧化氮的神经元的步骤。同时也评估了血管活性肠肽和一氧化氮合酶在肠道所有区域肌间神经丛中的共定位情况。结果表明，在所检查的胃肠道所有区域中均存在对蛋白基因产物、血管活性肠肽、P物质、降钙素基因相关肽、酪氨酸羟化酶、5-羟色胺和一氧化氮合酶呈免疫反应的神经纤维和神经细胞体。含有上述标志物的免疫反应性神经纤维和神经细胞体的分布模式在肠道的不同区域有所不同。然而，对一氧化氮合酶呈免疫反应且具有还原型辅酶Ⅱ黄递酶反应性的肌间神经元和神经纤维在整个肠道中的分布是相同的。与一些关于豚鼠和大鼠的研究不同，血管活性肠肽和一氧化氮合酶仅在一小部分肌间神经元中共存。
