Moulton J E, Sollod A E
Am J Vet Res. 1976 Jul;37(7):791-802.
In 7 calves inoculated with Trypanosoma brucei and observed for 105 days, the minimal clinical signs were occasional lethargic periods associated with parasitemia. Rectal temperatures increased by postinoculation day (pid), 10, remained moderately high until PID 50 to 60, and then returned to base line. Moderate anemia occurred between PID 15 and 25. Slightly increased lymphocyte count with relative decrease in neutrophil count was found in 3 calves. Monocytosis of minimal degree occurred 14 days after parasitemia. Trypanosome counts of low level and irregular frequency were found throughout the infection. The average number of trypanosomes in blood was approximately 1,000/ml. Antibody titers, as determined by the indirect immunofluorescent antibody method, appeared on PID 22 and stayed at moderate levels. The immunoglobulin M first increased at PID 5 and remained high (av 216% of base line value) throughout infection, but immunoglobulin G was never increased. Histopathologic study revealed proliferative changes in the lymph nodes and spleen characteristic of humoral antibody (B-cell) response. Mononuclear cell infiltration (lymphocytes and plasma cells), eosinophols, and edema were common in the lymph nodes, spleen, liver, heart, brain, hypophysis, testes, kidney, skeletal muscle, adrenal gland, pancreas, and uterus.
在7头接种布氏锥虫并观察105天的小牛中，最小的临床症状是偶尔出现与寄生虫血症相关的嗜睡期。接种后第10天直肠温度升高，直到接种后第50至60天一直保持中度高温，然后恢复到基线。接种后第15至25天出现中度贫血。3头小牛出现淋巴细胞计数略有增加而中性粒细胞计数相对减少的情况。寄生虫血症14天后出现轻度单核细胞增多。在整个感染过程中发现锥虫计数水平低且频率不规则。血液中锥虫的平均数量约为1000/ml。通过间接免疫荧光抗体法测定的抗体滴度在接种后第22天出现并保持在中度水平。免疫球蛋白M在接种后第5天首次升高，并在整个感染过程中保持高水平（平均为基线值的216%），但免疫球蛋白G从未升高。组织病理学研究显示淋巴结和脾脏出现体液抗体（B细胞）反应特有的增殖性变化。单核细胞浸润（淋巴细胞和浆细胞）、嗜酸性粒细胞和水肿在淋巴结、脾脏、肝脏、心脏、大脑、垂体、睾丸、肾脏、骨骼肌、肾上腺、胰腺和子宫中很常见。
