Goffin V, Piérard G E, Henry F, Letawe C, Maibach H I
Belgian SSTC Research Center, Department of Dermatopathology, University Medical Center Sart Tilman, Liège, Belgium.
Ecotoxicol Environ Saf. 1997 Aug;37(3):199-202. doi: 10.1006/eesa.1997.1537.
Interactions between bleaching agents containing sodium hypochlorite (NaOCl) and human stratum corneum are complex and not fully understood. The same applies when NaOCl is used as a war gas decontaminant. In this study data yielded by in vivo testing and an ex vivo bioassay are compared. Fifteen volunteers received patch tests of a neat proprietary NaOCl bleaching agent for 15, 30, 45, 60, and 90 min. No clinical reaction was seen. Reflectance colorimetry, transepidermal water loss (TEWL), and capacitance were measured for 72 hr after patch removal. Squamometry was also performed using D-Squames and colorimetry of the samples. In addition, the ex vivo corneoxenometry bioassay was conducted on various dilutions of the bleach. Data reveal that conductance and squamometry were more sensitive than TEWL to disclose the action of bleach upon the stratum corneum. Corneoxenometry proved to be a good predictive ex vivo bioassay, indicating the same information as the in vivo tests. Both squamometry and corneoxenometry appear valuable and complementary in assessing infraclinical damages of human skin by a NaOCl bleaching agent.
含次氯酸钠（NaOCl）的漂白剂与人体角质层之间的相互作用很复杂，尚未完全了解。当NaOCl用作战争毒气净化剂时也是如此。在本研究中，对体内测试和体外生物测定产生的数据进行了比较。15名志愿者接受了一种纯的专利NaOCl漂白剂的斑贴试验，时间分别为15、30、45、60和90分钟。未观察到临床反应。在去除斑贴后72小时测量反射比色法、经表皮水分流失（TEWL）和电容。还使用D-Squames进行了鳞屑测量和样品比色法。此外，对漂白剂的各种稀释液进行了体外角质层活力测定生物测定。数据显示，电导率和鳞屑测量比TEWL对揭示漂白剂对角质层的作用更敏感。角质层活力测定被证明是一种很好的预测性体外生物测定，与体内测试显示相同的信息。在评估NaOCl漂白剂对人体皮肤的亚临床损伤方面，鳞屑测量和角质层活力测定似乎都很有价值且相辅相成。
