TYB-2285及其代谢产物对体外淋巴细胞反应的影响。

The effects of TYB-2285 and its metabolites on lymphocyte responses in vitro.

Tominaga T, Watanabe A, Tsuji J, Koda A, Nagai H, Kumazawa Y, Shimada H

Department of Pharmacology, Toyobo Co. Ltd., Ohtsu Shiga, Japan.

Gen Pharmacol. 1997 Sep;29(3):473-5. doi: 10.1016/s0306-3623(96)00463-6.

Abstract

We examined the effects of TYB-2285 and its metabolites (TC-286 and TC-326) on antigen-induced lymphocyte proliferation, allogeneic mixed lymphocyte reaction (MLR) and mitogen-induced lymphocyte proliferation. 2. Splenic lymphocytes from C57BL/6 strain mice sensitized with human serum albumin were cultured with the antigen in the presence of TYB-2285 or its metabolites (10(-7)-10(-4) M). The lymphocyte proliferation enhanced by antigen was inhibited by TYB-2285 and its metabolites in a dose-dependent manner. 3. Splenic lymphocytes collected from C57BL/6 and Balb/C strain mice were cocultured in the presence of TYB-2285 and its metabolites (10(-7)-10(-4) M). Allogeneic MLR was inhibited by TYB-2285 and its metabolites in a dose-dependent manner. 4. Splenic lymphocytes from C47BL/6 strain mice were cultured with concanavalin A (Con A) in the presence of TYB-2285 and its metabolites (10(-7)-10(-4) M). TYB-2285 and its metabolites did not affect Con A-induced lymphocyte proliferation. Cyclosporin A showed an inhibitory effect on Con A-induced lymphocyte proliferation that was as strong as the inhibitory effect on antigen-induced lymphocyte proliferation and allogeneic MLR at a lower concentration.

摘要

我们研究了TYB - 2285及其代谢产物（TC - 286和TC - 326）对抗原诱导的淋巴细胞增殖、同种异体混合淋巴细胞反应（MLR）以及有丝分裂原诱导的淋巴细胞增殖的影响。2. 用人类血清白蛋白致敏的C57BL/6品系小鼠的脾淋巴细胞，在存在TYB - 2285或其代谢产物（10⁻⁷ - 10⁻⁴ M）的情况下与抗原一起培养。抗原增强的淋巴细胞增殖被TYB - 2285及其代谢产物以剂量依赖的方式抑制。3. 从C57BL/6和Balb/C品系小鼠收集的脾淋巴细胞，在存在TYB - 2285及其代谢产物（10⁻⁷ - 10⁻⁴ M）的情况下共培养。同种异体MLR被TYB - 2285及其代谢产物以剂量依赖的方式抑制。4. C47BL/6品系小鼠的脾淋巴细胞在存在TYB - 2285及其代谢产物（10⁻⁷ - 10⁻⁴ M）的情况下与伴刀豆球蛋白A（Con A）一起培养。TYB - 2285及其代谢产物不影响Con A诱导的淋巴细胞增殖。环孢素A对Con A诱导的淋巴细胞增殖有抑制作用，在较低浓度时，其抑制作用与对抗原诱导的淋巴细胞增殖和同种异体MLR的抑制作用一样强。