Makarova K S
Genetika. 1997 Jul;33(7):1016-9.
Sequence analysis of a 5' terminal fragment of stalker retrotransposon, about 1600 bp in length, revealed a number of motifs in the nucleotide sequences of the LTR and other untranslated regions that are common to retrotransposons 412 and MDG1. Taken together, experimental data and computer analysis of this sequence suggest that stalker falls into the gypsy group of retrotransposons and most closely resembles related retrotransposons 412 and MDG1. Stalker bears a small open reading frame (sORF) that shares 24 and 75% identical amino acid residues with the second sORFs of retrotransposons 412 and MDG1, respectively. Thus high similarity between the discovered sORF of stalker and the corresponding frame of MDG1 looks intriguing, since other regions of the sequences display little resemblance. Therefore, the evolutionary proximity of stalker to MDG1 cannot be conjectured solely on the basis of the mentioned similarity. The similarity may be explained by recombination of the two transposons, or, alternatively, the frame can retain its function in the genomes of stalker and MDG1 and lose it in 412.
对长度约为1600 bp的“追踪者”逆转录转座子5'末端片段进行序列分析，结果显示在长末端重复序列（LTR）和其他非翻译区的核苷酸序列中存在一些逆转录转座子412和MDG1共有的基序。综合实验数据和对该序列的计算机分析表明，“追踪者”属于逆转录转座子的吉普赛族，与相关逆转录转座子412和MDG1最为相似。“追踪者”带有一个小开放阅读框（sORF），分别与逆转录转座子412和MDG1的第二个sORF具有24%和75%的相同氨基酸残基。因此，“追踪者”中发现的sORF与MDG1的相应框架之间的高度相似性看起来很有趣，因为序列的其他区域几乎没有相似之处。所以，不能仅根据上述相似性推测“追踪者”与MDG1在进化上的亲缘关系。这种相似性可能是由两个转座子的重组来解释，或者，该框架在“追踪者”和MDG1的基因组中可以保留其功能，而在412中失去该功能。
