Avedisov S N, Il'in Iu V
Genetika. 1995 Jun;31(6):753-8.
The complete nucleotide sequence of the 7K variant of the gypsy retrotransposon of Drosophila melanogaster was determined. This variant belongs to the 7K subfamily of gypsy, which was previously considered inactive. All differences found in the sequenced 7K copy compared to the transpositionally active 6K variants were point mutations. These nucleotide substitutions account for about 1% of the total base pair number of gypsy. Long terminal repeats (LTR) have the highest rate of nucleotide substitutions However, changes in nontranslated regions did not involve the promoter region and other supposed cis-acting elements. Sixteen amino acid substitutions were found in the coding region of gypsy. These substitutions were mainly located on the boarders of potential functional domains and within the third open reading frame (ORF3). Comparative analysis of structures of these two variants of gypsy suggests the potential ability of 7K copies to transpose.
测定了黑腹果蝇吉普赛逆转座子7K变体的完整核苷酸序列。该变体属于吉普赛7K亚家族，此前被认为是无活性的。与具有转座活性的6K变体相比，在测序的7K拷贝中发现的所有差异均为点突变。这些核苷酸替换约占吉普赛总碱基对数的1%。长末端重复序列（LTR）的核苷酸替换率最高。然而，非翻译区的变化并未涉及启动子区域和其他假定的顺式作用元件。在吉普赛的编码区发现了16个氨基酸替换。这些替换主要位于潜在功能域的边界以及第三个开放阅读框（ORF3）内。对吉普赛这两个变体的结构进行比较分析表明，7K拷贝具有潜在的转座能力。
