Ptitsyn L R, Riazanova L A, Ivanov V B
Genetika. 1997 Jul;33(7):1020-4.
The bioluminescence method of assessing SOS response in Escherichia coli cells was applied to test the genotoxicity of five complex platinum compounds: cis-diamminedichloroplatinum, cycloplatam, trans-diamminedichloroplatinum, and two trans-binuclear complexes. Strains AB1157 (pPLS-1) and JC9239 (pPLS-1), a variant of AB1157 carrying the recF143 mutation, were used in the test procedure. SOS response in JC9239 cells was shown to be induced by significantly lower concentrations of cis-DDP and cycloplatam than in AB1157 cells. A drastic increase in cis-DDP toxicity for the JC9239 strain was shown. However, the presence of the recF mutation in JC9239 cells retarded the SOS response induced by trans-platinum compounds. The results obtained indicated that the E. coli recF system plays an essential role in repair and utilization of cis-DDP-DNA adducts.
采用评估大肠杆菌细胞中SOS反应的生物发光方法，来测试五种复杂铂化合物的遗传毒性：顺二氯二氨铂、环铂、反二氯二氨铂以及两种反式双核配合物。测试过程中使用了菌株AB1157（pPLS-1）和JC9239（pPLS-1），后者是携带recF143突变的AB1157变体。结果显示，与AB1157细胞相比，JC9239细胞中SOS反应可由浓度显著更低的顺铂和环铂诱导产生。结果表明，顺铂对JC9239菌株的毒性急剧增加。然而，JC9239细胞中recF突变的存在阻碍了反式铂化合物诱导的SOS反应。所得结果表明，大肠杆菌recF系统在顺铂-DNA加合物的修复和利用中起着至关重要的作用。
