Chen Y J, Chen P H, Lee M D, Chang J G
Department of Surgery, Taipei Medical College Hospital, Taiwan, Republic of China.
Int J Cancer. 1997 Sep 17;72(6):955-8. doi: 10.1002/(sici)1097-0215(19970917)72:6<955::aid-ijc6>3.0.co;2-o.
The FHIT gene was recently discovered and proposed as a tumor-suppressor gene. We examined the FHIT gene in a panel of digestive-tract cancers along with their corresponding non-tumorous tissues by reverse transcription of FHIT mRNA followed by PCR amplification and sequencing of the products. A normal FHIT transcript was found to be expressed at robust levels in 44 of 46 cancerous tissues and in all of 46 non-cancerous tissues tested. Of the 46 cancerous specimens, 21 (45.7%) revealed the occurrence of aberrant transcripts. In addition, of the 46 matched normal tissues, 14 (30.4%) showed the existence of aberrant transcripts. Sequence analysis confirmed that aberrant transcripts were missing in one or more exons of the FHIT cDNA, while 8 cases displayed aberrant transcripts of the FHIT gene both in cancerous and in non-cancerous tissues. However, sequence analysis revealed that the patterns of the aberrant transcripts were different between the corresponding paired samples. In addition, there were 6 cases displaying aberrant FHIT transcripts only in their normal-tissue counterparts. These studies indicate that abnormalities of the FHIT gene transcripts occur at a fairly high frequency in cancerous and corresponding non-cancerous lesions of the digestive tract. However, they might not be causally related to the tumorigenesis of the digestive tract.
FHIT基因最近被发现并被认为是一种肿瘤抑制基因。我们通过逆转录FHIT mRNA，然后对产物进行PCR扩增和测序，在一组消化道癌及其相应的非肿瘤组织中检测了FHIT基因。在46个癌组织中的44个以及所有46个检测的非癌组织中，发现正常的FHIT转录本以较高水平表达。在46个癌标本中，21个（45.7%）显示存在异常转录本。此外，在46个匹配的正常组织中，14个（30.4%）显示存在异常转录本。序列分析证实，异常转录本在FHIT cDNA的一个或多个外显子中缺失，而8例在癌组织和非癌组织中均显示FHIT基因的异常转录本。然而，序列分析显示相应配对样本之间异常转录本的模式不同。此外，有6例仅在其正常组织对应物中显示异常的FHIT转录本。这些研究表明，FHIT基因转录本的异常在消化道癌及其相应的非癌病变中出现的频率相当高。然而，它们可能与消化道肿瘤的发生没有因果关系。
