Sudhoff H, Borkowski G, Bujia J, Hildmann H, Fisseler-Eckhoff A
Hals-Nasen-Ohrenklinik, Ruhr-Universität Bochum, St. Elisabeth Hospital.
HNO. 1997 Aug;45(8):630-5. doi: 10.1007/s001060050138.
The development of a middle ear cholesteatoma is usually associated with chronic inflammation and displacement of the mucosa present by the invading squamous epithelium. To analyze the clinically different behaviors of both epithelia, we used immunohistochemical methods to study the distribution and expression of interleukin-1 (Il-1), transforming growth factor-alpha (TGF-alpha), epidermal growth factor (EGF), epidermal growth factor-receptor (EGF-R), the proliferation marker MIB 1, c-myc proto-oncogene product and activation marker 4F2. Results stromal that keratinocytes in a cholesteatoma exhibited a much higher activation and proliferation rate when compared to middle ear mucosa cells. Middle ear epithelial cells showed no immunoreactivity for TGF-alpha, EGF-R, Il-1 and c-myc in contrast to the markedly positive immunoreactivity found in cholesteatoma matrix. The local release of cytokines and growth factors, such as TGF-alpha, EGF and Il-1 by inflammatory cells seems to be an important factor for the hyper-proliferative behavior of cholesteatoma epithelium. Our findings could contribute to the pathogenesis of middle ear cholesteatoma and give a possible explanation for the sustained progression of its growth leading to displacement of the middle ear mucosa.
中耳胆脂瘤的形成通常与慢性炎症以及侵袭性鳞状上皮导致的黏膜移位有关。为了分析两种上皮细胞在临床上的不同行为，我们采用免疫组织化学方法研究白细胞介素 -1（Il-1）、转化生长因子 -α（TGF-α）、表皮生长因子（EGF）、表皮生长因子受体（EGF-R）、增殖标志物MIB 1、c-myc原癌基因产物和激活标志物4F2的分布及表达。结果显示，与中耳黏膜细胞相比，胆脂瘤中的角质形成细胞表现出更高的激活和增殖率。与胆脂瘤基质中明显阳性的免疫反应性相比，中耳上皮细胞对TGF-α、EGF-R、Il-1和c-myc没有免疫反应性。炎症细胞局部释放细胞因子和生长因子，如TGF-α、EGF和Il-1，似乎是胆脂瘤上皮细胞过度增殖行为的一个重要因素。我们的研究结果可能有助于阐明中耳胆脂瘤的发病机制，并为其持续生长导致中耳黏膜移位提供一个可能的解释。
