Murata M, Nakagawa M, Takahashi S
Department of Ophthalmology, Yamagata University School of Medicine, Japan.
Ophthalmologica. 1997;211(6):384-6. doi: 10.1159/000310835.
Angiotensin II type 1 receptor (AT1) is expressed in a variety of tissues. However, the localization of the specific AT1 mRNA in several ocular tissues has not been fully elucidated. In the present study, we investigated the expression and distribution of AT1 mRNA in the several ocular tissues of Wistar-Kyoto rats (WKYs) using the technique of reverse transcription with polymerase chain reaction. The 5 cornea, sclera, iris, ciliary body, and choroid samples tested were negative. When we analyzed 5 retina samples, 4 were positive. AT1 mRNA was more significantly expressed in the retina of WKYs than in the other ocular tissues of WKYs (p = 0.0238 < 0.05). Our study provides the first direct evidence that the AT1 is abundantly present in the WKY retina portions. This is a strategic location for an ocular renin-angiotensin system to influence retinal cicrulation.
血管紧张素II 1型受体（AT1）在多种组织中表达。然而，特定AT1 mRNA在几种眼组织中的定位尚未完全阐明。在本研究中，我们使用聚合酶链反应逆转录技术，研究了Wistar-Kyoto大鼠（WKYs）几种眼组织中AT1 mRNA的表达和分布。所检测的5个角膜、巩膜、虹膜、睫状体和脉络膜样本均为阴性。当我们分析5个视网膜样本时，4个为阳性。WKYs视网膜中AT1 mRNA的表达比WKYs的其他眼组织更显著（p = 0.0238 < 0.05）。我们的研究提供了首个直接证据，证明AT1大量存在于WKY视网膜部分。这是眼肾素-血管紧张素系统影响视网膜循环的一个关键位置。
