蛋白质逐步展开。
Proteins unfold in steps.
作者信息
Zocchi G
机构信息
Center for Chaos and Turbulence Studies, Niels Bohr Institute, Blegdamsvej 17, 2100 Copenhagen O, Denmark.
出版信息
Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10647-51. doi: 10.1073/pnas.94.20.10647.
Abstract
I present results from an experiment on the dynamics of folding of a globular protein (bovine serum albumin). Employing a micro-mechanical technique, I perform the measurements on very few molecules (1-100). I observed a sequence of steps in time for both unfolding and refolding. The overall characteristic time of the process is thus built up of waiting times between successive steps. The pattern of steps is reproducible, demonstrating the existence of deterministic pathways for folding and unfolding. Certain symmetries in the patterns of steps may reflect the architecture of the protein's structure.
摘要
我展示了一项关于球状蛋白（牛血清白蛋白）折叠动力学实验的结果。采用微机械技术，我对极少数分子（1 - 100个）进行了测量。我观察到了蛋白质展开和重新折叠过程中一系列随时间变化的步骤。因此，该过程的整体特征时间是由连续步骤之间的等待时间构成的。这些步骤的模式是可重复的，这表明折叠和展开过程存在确定性的路径。步骤模式中的某些对称性可能反映了蛋白质结构的架构。
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