Klechikov V Z
Arkh Patol. 1976 Apr;38(4):86-8.
The article discusses results of the investigation into variability of the thickness of sections of a non-fixed frozen tissue obtained in cryostat, the dependence of optic density of histoenzymological preparations upon the thickness of a section and duration of incubation, degree of the accuracy of measurements of the optic density by probes of a different diameter, compatibility of results obtained by various techniques of cytospectrophotometry. It is shown that when the scale of microtome-cryostat was set at 5 mcm the thickness of sections varied in the range of 10%, at 10 mcm--in the range of 2%. In preparations as thick as 10 mcm2 and more lineal interrelationships between their optic density and duration of incubation were observed. In the photometric analysis of results of tetrasole reactions the accuracy of measurement is inversely proportional to the size of the probe: M+/-m--0.27+/-0.006--0.009 with area 3.0 mcm2 and 0.27+/-0.002 fith aea 0.8 mcm2. Results of measurement of optic density of ethanol by photoelectric and photographic techniques of cytospectrophometry (on the apparatus MUF-5, MUF-6, MF-2, MF-4, IFO-451) differ in the range of 0.5%, of histoenzymalogical preparations--in the range of 5--7%. On the basis of the data obtained a conclusion may be drawn that, if certain conditions are observed, the cytospectrophotometric analysis of histoenzymological preparations will give compatible results the accuracy of which meets the requirements put in a 95% level of probability.
本文讨论了关于在低温恒温器中获得的非固定冷冻组织切片厚度变异性的研究结果、组织酶学制剂的光密度与切片厚度及孵育时间的依赖性、不同直径探针测量光密度的准确度、各种细胞分光光度法技术所得结果的兼容性。结果表明，当切片机 - 低温恒温器的刻度设置为5微米时，切片厚度的变化范围为10%，设置为10微米时，变化范围为2%。在厚度为10微米及以上的制剂中，观察到其光密度与孵育时间之间存在线性关系。在四氮唑反应结果的光度分析中，测量准确度与探针尺寸成反比：对于面积为3.0平方厘米的探针，M±m为0.27±0.006 - 0.009，对于面积为0.8平方厘米的探针，M±m为0.27±0.002。用细胞分光光度法的光电和摄影技术（在MUF - 5、MUF - 6、MF - 2、MF - 4、IFO - 451仪器上）测量乙醇光密度的结果差异在0.5%范围内，测量组织酶学制剂光密度的结果差异在5 - 7%范围内。根据所得数据可以得出结论：如果遵守某些条件，对组织酶学制剂进行细胞分光光度分析将得到兼容的结果，其准确度符合95%概率水平的要求。
