Mouneimne H, Juvin M, Beretti J L, Azoulay-Dupuis E, Vallee E, Geslin P, Petitpretz P, Berche P, Gaillard J L
Laboratoire de Microbiologie, INSERM U411, Faculté Necker-Enfants Malades, Paris, France.
Clin Diagn Lab Immunol. 1997 Nov;4(6):778-82. doi: 10.1128/cdli.4.6.778-782.1997.
To detect new antigen candidates for serological tests, we studied the antibody response to pneumococcal protein antigens in mice infected intratracheally with various Streptococcus pneumoniae strains. Sera were tested by Western blotting against whole-cell protein extracts. Mice developed a detectable immunoglobulin G-type response against a small number of polypeptides. The antibody response was strain dependent: sera from individuals infected with the same strain gave similar banding patterns on immunoblots. The banding patterns varied with the strain used for infection. However, a band at 36 to 38 kDa was recognized by all reactive sera. This band appeared to correspond to a polypeptide that was antigenically well conserved among the different S. pneumoniae serotypes. An antibody response to this antigen developed in mice irrespective of the capsular type, the virulence, and the susceptibility to penicillin G of the infecting strain. Thus, this 36- to 38-kDa protein antigen may be of value for the development of a serological test for humans.
为了检测血清学检测的新抗原候选物，我们研究了经气管内感染各种肺炎链球菌菌株的小鼠对肺炎球菌蛋白抗原的抗体反应。通过蛋白质印迹法用全细胞蛋白提取物检测血清。小鼠针对少数几种多肽产生了可检测到的免疫球蛋白G型反应。抗体反应具有菌株依赖性：感染同一菌株的个体的血清在免疫印迹上呈现相似的条带模式。条带模式随用于感染的菌株而变化。然而，所有反应性血清都识别出一条36至38 kDa的条带。这条带似乎对应于一种在不同肺炎链球菌血清型中抗原性高度保守的多肽。无论感染菌株的荚膜类型、毒力和对青霉素G的敏感性如何，小鼠都会针对这种抗原产生抗体反应。因此，这种36至38 kDa的蛋白质抗原可能对开发人类血清学检测有价值。
