Coonrod J D, Rylko-Bauer B
Infect Immun. 1977 Dec;18(3):617-23. doi: 10.1128/iai.18.3.617-623.1977.
Previous studies of complement-fixing antibodies to pneumococcal capsular polysaccharides in humans have yielded conflicting results. We studied 65 sera from 25 patients with pneumococcal pneumonia, using both fresh sera and heat-inactivated sera with added human complement. Only 4 of the 25 patients developed detectable levels of complement-fixing anticapsular antibody. Of the 25 patients, 22 developed detectable levels of hemagglutinating anticapsular antibody, indicating that they were able to develop an immunological response during the infection. Most of the antibody detected by hemagglutination was sensitive to 2-mercaptoethanol, but some 2-mercaptoethanol-resistant antibody was also detected. In studies with rabbit antiserum, the complement fixation test was found to be as sensitive as the hemagglutination test for detection of anticapsular antibody. It is not clear why detectable levels of complement-fixing antibody do not develop more often in patients with pneumococcal pneumonia. Studies of purified anticapsular antibody would be of interest to determine whether or not these antibodies are restricted to immunoglobulin subclasses having a limited capacity to fix complement.
先前关于人类针对肺炎球菌荚膜多糖的补体结合抗体的研究结果相互矛盾。我们使用新鲜血清和添加了人补体的热灭活血清，对25例肺炎球菌肺炎患者的65份血清进行了研究。25例患者中只有4例产生了可检测水平的补体结合抗荚膜抗体。25例患者中有22例产生了可检测水平的血凝抗荚膜抗体，这表明他们在感染期间能够产生免疫反应。通过血凝检测到的大多数抗体对2-巯基乙醇敏感，但也检测到了一些对2-巯基乙醇有抗性的抗体。在对兔抗血清的研究中，发现补体结合试验在检测抗荚膜抗体方面与血凝试验一样敏感。目前尚不清楚为什么肺炎球菌肺炎患者中可检测水平的补体结合抗体没有更频繁地产生。对纯化的抗荚膜抗体进行研究，以确定这些抗体是否仅限于补体结合能力有限的免疫球蛋白亚类，将会很有意义。
