Frank H L, Hemker H C
Basic Res Cardiol. 1976 May-Jun;71(3):252-62.
Perfusion of the isolated rat heart with a fluid containing 800mM glycerol in addition to its normal constituents causes a contraction height decrease of about 50%. A change to normal perfusion fluid causes a period of contracture to occur. After this period the heart is perfectly viable. Mechanical and osmotic effects could be ruled out as possible causes. No gross disturbances were seen in the electron micrographs. An effect on intermediary metabolism is equally unlikely. Presumably, we must seek the explanation in a direct effect of the glycerol on the Ca++ fluxes across the cellular and intracellular membranes.
用一种除了含有正常成分外还含有800mM甘油的液体灌注离体大鼠心脏，会导致收缩高度下降约50%。换成正常灌注液会引发一段挛缩期。在此期间过后，心脏完全可以存活。可以排除机械和渗透作用作为可能的原因。电子显微镜照片中未发现明显异常。甘油对中间代谢产生影响的可能性同样不大。据推测，我们必须从甘油对跨细胞膜和细胞内膜的Ca++通量的直接作用中寻找解释。
