Wipat A, Carter N, Brignell S C, Guy B J, Piper K, Sanders J, Emmerson P T, Harwood C R
School of Microbiological, Immunological and Virological Sciences, University of Newcastle upon Tyne, UK.
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3067-78. doi: 10.1099/13500872-142-11-3067.
Within the framework of the international programme to sequence the genome of Bacillus subtilis strain 168, we were allocated the region between dnaB (256 degrees) and pheA (240 degrees). The sequencing of this region is now complete and we report our primary analysis of the 114 kb region containing 114 ORFs. In addition to previously characterized genes, we have identified genes involved in the utilization of plant cell wall polysaccharides, stress responses and the metabolism of amino acids, cell walls, DNA and fatty acids. We also discuss various structural and physical features, including the orientation of genes with respect to replication, putative start and stop codons, ribosome binding sites and rho-independent transcription terminators.
在对枯草芽孢杆菌168菌株基因组进行测序的国际项目框架内，我们负责对dnaB（256°）和pheA（240°）之间的区域进行测序。该区域的测序现已完成，我们在此报告对这个包含114个开放阅读框（ORF）的114 kb区域的初步分析。除了先前已鉴定的基因外，我们还识别出了参与植物细胞壁多糖利用、应激反应以及氨基酸、细胞壁、DNA和脂肪酸代谢的基因。我们还讨论了各种结构和物理特征，包括基因相对于复制的方向、推定的起始和终止密码子、核糖体结合位点以及不依赖于rho因子的转录终止子。
