Trenerry V C, Ward C M
Australian Government Analytical Laboratories, South Australia, Australia.
J Capillary Electrophor. 1996 Sep-Oct;3(5):271-4.
A rapid method for the determination of quinine in bitter drinks by micellar electrokinetic capillary chromatography (MECC) with ultraviolet (UV) detection is described. The beverage is simply diluted with deionized water, filtered, and analyzed using a 75 cm x 75 micrometer uncoated fused-silica capillary column with a buffer consisting of 15% methanol and 85% of a mixture of 0.05 M cetyltrimethylammonium bromide (CTAB), 0.01 M sodium tetraborate, and 0.01 M potassium dihydrogen orthophosphate, pH 8.6, with an operating voltage of -25 kV. The levels of quinine determined by MECC were in good agreement with those determined by HPLC. The CVs for area calculation (2.1%, n = 7) and migration time variation (1.3%, n = 20) for multiple injections of a sample solution by MECC were satisfactory.
描述了一种采用胶束电动毛细管色谱法（MECC）结合紫外（UV）检测来快速测定苦味饮料中奎宁的方法。饮料只需用去离子水稀释、过滤，然后使用一根75 cm×75 微米的未涂层熔融石英毛细管柱进行分析，缓冲液由15%甲醇和85%的混合溶液组成，该混合溶液包含0.05 M十六烷基三甲基溴化铵（CTAB）、0.01 M硼酸钠和0.01 M磷酸二氢钾，pH值为8.6，操作电压为 -25 kV。通过MECC测定的奎宁含量与通过高效液相色谱法（HPLC）测定的结果高度一致。MECC对样品溶液多次进样的面积计算变异系数（CVs，2.1%，n = 7）和迁移时间变化（1.3%，n = 20）令人满意。
