Colyer C L
Department of Chemistry, University of Alberta, Edmonton, Canada.
J Capillary Electrophor. 1996 May-Jun;3(3):131-7.
Emersion peaks, baseline shifts, and spontaneous-marker peaks in CE are documented. These baseline perturbations are observed in a simple CE system that does not employ sample injection and with sodium benzoate as the running electrolyte. Emersion peaks are attributed to the preferential adsorption of electrolyte at the air-solution interface formed upon emersion of the capillary inlet. Baseline shifts are believed to be caused by field-dependent changes in electrolyte concentration entering the capillary. These shifts may be accompanied by spontaneous-marker peaks, which may be caused by diffusion into or out of the capillary inlet between electrophoretic experiments. All baseline perturbations are detected as a change in UV absorbance signal and are transported by electroosmotic flow.
记录了毛细管电泳中的出峰、基线漂移和自发标记峰。在一个不采用进样且以苯甲酸钠作为运行电解质的简单毛细管电泳系统中观察到了这些基线扰动。出峰归因于毛细管入口露出时在气-液界面处电解质的优先吸附。基线漂移被认为是由进入毛细管的电解质浓度随电场变化所致。这些漂移可能伴随着自发标记峰，其可能是由于在电泳实验之间扩散进入或离开毛细管入口而引起的。所有基线扰动都作为紫外吸收信号的变化被检测到，并通过电渗流进行传输。
