Bihoreau N, Ramon C, Vincentelli R, Levillain J P, Troalen F
Department of Analytical Biochemistry, French Laboratory of Fractionation and Biotechnology, Les Ulis, France.
J Capillary Electrophor. 1995 Jul-Aug;2(4):197-202.
CE has been employed for peptide mapping characterization of the light chain of a human anti-Rhesus (D) (Rh[D]) antibody presently undergoing clinical evaluation. In the presence of an ion-pairing agent used to increase resolution, reproducible maps were obtained within 55 min after injection of 12 fmol of protein. CE has also been employed as a direct and quick screening tool for purity evaluation of the tryptic peptides obtained from reversed-phase high-performance liquid chromatography (RP-HPLC) prior to sequence analysis. Post-translational modifications of the protein were determined by identification of the cysteine residues implicated in disulfide linkages.
毛细管电泳（CE）已被用于对一种正在进行临床评估的人抗恒河猴（D）（Rh[D]）抗体轻链进行肽图谱表征。在使用离子对试剂以提高分离度的情况下，注入12飞摩尔蛋白质后55分钟内即可获得可重复的图谱。CE还被用作直接且快速的筛选工具，用于在序列分析之前对从反相高效液相色谱（RP-HPLC）获得的胰蛋白酶肽段进行纯度评估。通过鉴定参与二硫键连接的半胱氨酸残基来确定蛋白质的翻译后修饰。
