Dlouha A, Lecroisey A, Henschen A, Ruttyn Y, Rouger P, Keil B
Unité de Chimie des Proteines, Institut Pasteur, Paris, France.
Protein Seq Data Anal. 1991 Dec;4(6):319-24.
Antibody H2D5D2F5 is a human monoclonal anti-Rh(D) IgG1 (lambda) produced by Epstein-Barr virus-immortalized B lymphocytes from a healthy donor. The complete amino acid sequence of the light (L) chain, with the exception of positions 94-97, was determined by Edman degradation of the intact chain, containing 30 residues, and derived tryptic and thermolytic peptides. Sequences of the peptides were aligned by comparison with the sequences of previously reported L chains. H2D5D2F5 L chain belongs to the first variable subgroup of human chains. Its sequence does not reveal striking differences when compared to those of other human lambda chains issued from myeloma or hybridoma.
抗体H2D5D2F5是一种人源单克隆抗Rh(D) IgG1（λ型），由来自健康供体的爱泼斯坦-巴尔病毒永生化B淋巴细胞产生。轻链（L链）完整序列（除94 - 97位外）通过对包含30个残基完整链以及衍生的胰蛋白酶和嗜热菌蛋白酶肽段进行埃德曼降解来确定。通过与先前报道的L链序列比较对肽段序列进行比对。H2D5D2F5 L链属于人源链的第一个可变亚组。与骨髓瘤或杂交瘤来源的其他人源λ链相比，其序列未显示出显著差异。
