Najeme F, Julien J, Herblot S, Dousset V, Brochet B, Bonnet J
Laboratoire d'Immunologie Moléculaire, Bâtiment 1b-Carreire Nord, Université de Bordeaux II, France.
Brain Res Brain Res Protoc. 1997 May;1(2):133-8. doi: 10.1016/s1385-299x(96)00021-9.
Myelin basic proteins (MBPs) are a set of proteins making up about 30% of the protein content of the central nervous system myelin. Four human isoforms have been identified. The most abundant is a highly conserved 18.5 kDa polypeptide. For this species, the amino acid sequence homologies between human and monkey or human and chick are 98.2% and 71.1%, respectively. As a consequence, there is a very good immunological cross-reactivity between the mammalian MBP. This protein has been extensively used to induce experimental allergic encephalomyelits (EAE) in numerous animals. The evolution of chronic EAE in animal is similar to that of multiple sclerosis (MS), a demyelinating human pathology, and chronic EAE is considered to be an animal model of MS. In demyelinating pathologies, MBP concentration in the cerebrospinal fluid (CSF) is considered to be a good marker of demyelination. MBP concentration, in biological fluids, is generally determined by radioimmunoassay (RIA). The RIA technique currently used is highly sensitive (0.1-2.5 ng/ml) but has the drawback of requiring the handling of radioactivity and frequent labelling of MBP. So we developed a new enzyme immunoassay (EIA) technique. Our technique has the same sensitivity as RIA, needs only small volumes of CSF (50 microliters) and the enzyme-labelled MBP tracer is stable for at least 12 months.
髓鞘碱性蛋白（MBP）是一组蛋白质，约占中枢神经系统髓鞘蛋白质含量的30%。已鉴定出四种人类异构体。最丰富的是一种高度保守的18.5 kDa多肽。对于该物种，人类与猴子或人类与鸡之间的氨基酸序列同源性分别为98.2%和71.1%。因此，哺乳动物的MBP之间存在非常好的免疫交叉反应性。这种蛋白质已被广泛用于在多种动物中诱导实验性变应性脑脊髓炎（EAE）。动物慢性EAE的演变与脱髓鞘性人类疾病多发性硬化症（MS）相似，慢性EAE被认为是MS的动物模型。在脱髓鞘疾病中，脑脊液（CSF）中的MBP浓度被认为是脱髓鞘的良好标志物。生物体液中的MBP浓度通常通过放射免疫测定法（RIA）测定。目前使用的RIA技术高度灵敏（0.1 - 2.5 ng/ml），但有需要处理放射性以及频繁标记MBP的缺点。因此我们开发了一种新的酶免疫测定法（EIA）技术。我们的技术与RIA具有相同的灵敏度，仅需要少量脑脊液（50微升），并且酶标记的MBP示踪剂至少稳定12个月。
