Fiore J R, La Grasta L, Di Stefano M, Buccoliero G, Pastore G, Angarano G
Clinic of Infectious Diseases, University of Bari, Italy.
New Microbiol. 1997 Oct;20(4):339-44.
Evidence indicates that seminal plasma is cytotoxic when used in lymphocyte cultures. In fact, an amine oxidase which is normally present in Fetal Calf Serum (FCS) causes spermine oxidation and, finally, cytotoxic substances are released. This seminal quality might obviously hamper studies aiming to evaluate the role of semen in HIV sexual transmission, since lymphocytes are generally used as target or effector cells in virological or immunological studies on HIV. We evaluated the efficacy of FCS free-medium, heat inactivated seminal plasma and/or the washing-out of cultures after a three hour incubation period in preventing the cytotoxicity to lymphocytes. Results show that when cultures are carried out in the absence of FCS and/or after seminal plasma removal, cytotoxicity is not prevented, although delayed. This finding indicates that, along with still unrecognized additional factors, spermine oxidation is still an important factor for semen cytotoxicity, and this considerably hampers any immunological and virological study, requiring the use of lymphocytes, concerning seminal fluid.
有证据表明，精浆用于淋巴细胞培养时具有细胞毒性。事实上，胎牛血清（FCS）中通常存在的一种胺氧化酶会导致精胺氧化，最终释放出细胞毒性物质。这种精液特性显然可能会阻碍旨在评估精液在HIV性传播中作用的研究，因为在关于HIV的病毒学或免疫学研究中，淋巴细胞通常用作靶细胞或效应细胞。我们评估了无FCS培养基、热灭活精浆和/或在三小时孵育期后冲洗培养物在预防对淋巴细胞的细胞毒性方面的效果。结果表明，当在无FCS的情况下进行培养和/或去除精浆后，虽然细胞毒性会延迟，但并不能预防。这一发现表明，除了尚未识别的其他因素外，精胺氧化仍是精液细胞毒性的一个重要因素，这严重阻碍了任何涉及精液且需要使用淋巴细胞的免疫学和病毒学研究。
