Bergmann M, Post A, Rittel I, Bechmann I, Nitsch R
Institut für Anatomie und Spezielle Embryologie, Universität Fribourg, Switzerland.
Exp Brain Res. 1997 Oct;117(1):80-6. doi: 10.1007/s002210050201.
Expression of the synaptic vesicle protein synaptophysin was studied in lesion-induced sprouting neurons of the contralateral entorhinal cortex and in the contralateral dentate gyrus using immunocytochemistry at the light- and electron-microscopic level. Perikaryal immunoreactivity for synaptophysin was found between 8 and 10 days postlesion. Light microscopy revealed that synaptophysin immunostaining was present in almost all neurons of layers II and III of the contralateral medial entorhinal cortex. These neurons give rise to the sprouting, crossed temporodentate pathway. In addition, some hilar neurons of the contralateral dentate gyrus, which are the parent cells of sprouting commissural fibers, were immunostained for synaptophysin. Transient immunostaining for synaptophysin was observed within cell bodies and dendrites. Additionally, the cell bodies were outlined by immunoreactive puncta, identified by electron microscopy as nerve terminals. Our results revealed that sprouting neurons express the major synaptic vesicle protein synaptophysin during reactive synaptogenesis in a pattern that reflects biosynthesis and sorting of this protein as seen in developing neurons during synapse formation.
利用免疫细胞化学方法，在光镜和电镜水平上研究了突触囊泡蛋白突触素在对侧内嗅皮质损伤诱导的发芽神经元和对侧齿状回中的表达。在损伤后8至10天发现了突触素的胞体免疫反应性。光镜显示，对侧内侧内嗅皮质II层和III层的几乎所有神经元中都存在突触素免疫染色。这些神经元产生发芽的、交叉的颞齿状通路。此外，对侧齿状回的一些海马神经元，即发芽联合纤维的母细胞，也被突触素免疫染色。在细胞体和树突内观察到突触素的短暂免疫染色。此外，细胞体被免疫反应性小点勾勒出来，电镜鉴定为神经末梢。我们的结果表明，在反应性突触形成过程中，发芽神经元表达主要的突触囊泡蛋白突触素，其模式反映了这种蛋白在突触形成过程中发育中的神经元中所见的生物合成和分选。
