Sherman W V, Korenstein R, Caplan S R
Biochim Biophys Acta. 1976 Jun 8;430(3):454-8. doi: 10.1016/0005-2728(76)90021-9.
Arrhenius parameters for formation and decay of phototransients in suspensions of purple membrane fragments in H2O and 2H2O have been determined in the temperature range 0-60 degree C. Kinetic isotope effects are found which show that proton transfer steps are involved in both formation and decay of the two longest-lived transients absorbing at 410 nm and 660 nm, respectively. The results also suggest that these transients do not occupy a single pathway in the spontaneous deexcitation of bacteriorhodopsin within the purple membrane. Purple membrane undergoes a phase transition at 25-30 degrees C in both H2O and 2H2O.
已测定了紫色膜片段悬浮于水和重水中时，光瞬态形成和衰减的阿伦尼乌斯参数，测定温度范围为0至60摄氏度。发现了动力学同位素效应，这表明质子转移步骤分别参与了在410纳米和660纳米处吸收的两个寿命最长的瞬态的形成和衰减。结果还表明，这些瞬态在紫色膜内细菌视紫红质的自发去激发过程中并非占据单一途径。在水和重水中，紫色膜在25至30摄氏度时都会发生相变。
