T7 DNA/噬菌体在叙利亚仓鼠胚胎细胞中的附着与穿透

The attachment and penetration of T7 DNA/phage in Syrian hamster embryonic cells.

作者信息

Leavitt J C, Schechtman L M, Ts'o P O, Borenfreund E, Bendich A

出版信息

Biochim Biophys Acta. 1976 Jun 18;435(2):167-83. doi: 10.1016/0005-2787(76)90248-3.

Abstract

Bacteriophage T7 DNA can penetrate Syrian hamster embryonic cells after a mandatory initial pretreatment with DEAE-dextran. In 3 h an extracellular complex between T7DNA and the cell monolayer is formed which is equivalent to 105 T7 genomes per cell. During the ensuing 24-48 h of cell growth, an average of 102-103 T7 genomes are transported to the nucleus in 90% of the cells of the culture.

摘要

在用DEAE-葡聚糖进行必要的初始预处理后，噬菌体T7 DNA可穿透叙利亚仓鼠胚胎细胞。在3小时内，T7 DNA与细胞单层之间形成细胞外复合物，相当于每个细胞有105个T7基因组。在随后细胞生长的24 - 48小时内，培养物中90%的细胞平均有102 - 103个T7基因组被转运到细胞核中。