噬菌体T7 DNA的标记拯救与部分复制
Marker rescue and partial replication of bacteriophage T7 DNA.
作者信息
Burck K B, Miller R C
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6144-8. doi: 10.1073/pnas.75.12.6144.
Abstract
Experiments reported here show that some UV-irradiated wild-type T7 phage markers can be rescued efficiently by coinfection with T7 amber mutant phage in a permissive host. Other results show that the segments of a UV-irradiated genome that replicate efficiently are those that also are rescued efficiently during a marker rescue experiment. At higher doses, fewer markers are rescued efficiently and fewer segments of the genome replicate efficiently. The results clearly indicate that the probability of marker rescue is correlated with the ability of the DNA containing the marker to replicate. Sucrose density gradient analysis shows that UV irradiation does not produce double-strand scissions in T7 DNA at doses used here. Therefore, the partial replication and rescue of markers from the left end of the genome is not due simply to injection of only the left end of the T7 DNA.
摘要
此处报道的实验表明，在允许性宿主中，一些经紫外线照射的野生型T7噬菌体标记物可通过与T7琥珀突变体噬菌体共感染而有效地得到拯救。其他结果表明，经紫外线照射的基因组中能有效复制的片段，也是在标记物拯救实验中能有效得到拯救的片段。在较高剂量下，能有效拯救的标记物较少，基因组中能有效复制的片段也较少。结果清楚地表明，标记物拯救的概率与含有该标记物的DNA的复制能力相关。蔗糖密度梯度分析表明，此处所用剂量的紫外线照射不会在T7 DNA中产生双链断裂。因此，基因组左端标记物的部分复制和拯救并非仅仅是由于只注入了T7 DNA的左端。
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