在大鼠血浆存在的情况下，雌二醇和孕酮与其抗体结合的参数。
The parameters of the binding of estradiol and progesterone to their antibodies in presence of rat plasma.
作者信息
Dessen P, Erdos T
出版信息
Biochimie. 1976;58(4):455-64. doi: 10.1016/s0300-9084(76)80256-8.
The binding of estradiol and progesterone to the corresponding antibodies has been studied in system containing : a) antibody and hormone or b) antibody, hormone and rat plasma. In sytem a) the apparent association constant and the concentration of the antibody have been determined and the optimal conditions of a standard antibody-assay have been elaborated. In system b) the specific binding of hormone to antibody in presence of rat plasma has been measured, and the value of the "Operational Association Constant", Kop, has been calculated. Kop has the dimensions of an apparent association constant : it defines the ratio hormone bound/unbound to antibody, as a function of hormone concentration in system b). Based upon these experiments, the extent of hormone-binding to antibody injected to rats can be predicted. When antibody and hormone concentrations are measured in the plasma, the concentrations of hormone bound to antibody can be calculated.
已在包含以下成分的体系中研究了雌二醇和孕酮与相应抗体的结合
a）抗体和激素，或b）抗体、激素和大鼠血浆。在体系a）中，已确定了表观缔合常数和抗体浓度，并阐述了标准抗体测定的最佳条件。在体系b）中，已测量了在大鼠血浆存在下激素与抗体的特异性结合，并计算了“操作缔合常数”Kop的值。Kop具有表观缔合常数的量纲：它定义了体系b）中结合/未结合抗体的激素比率与激素浓度的函数关系。基于这些实验，可以预测注射到大鼠体内的激素与抗体的结合程度。当测量血浆中的抗体和激素浓度时，可以计算出与抗体结合的激素浓度。
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