Guillon G, Massoulié J, Guillon G, Massoulié J
Biochimie. 1976;58(4):465-71. doi: 10.1016/s0300-9084(76)80257-x.
Multiple forms of acetylcholinesterase have been studied in Goldfish adapted to 15 degrees C and 25 degrees C. Three forms of enzyme (sedimenting at 18S, 12S and 5S) could be distinguished by sucrose gradient centrifugation. Their molecular and enzymatic properties did not vary with environmental temperature. It was noted that the relative proportion of the three forms differed between brain and muscle extracts, the 18S forms predominating in muscle (about 75 per cent) the 12S forms being more abundant in the brain, suggesting a physiological differentiation between these forms. The latter form varied from 48 per cent to 70 per cent in Goldfish adapted to 15 degrees and 25 degrees C respectively. The relative efficiency of saline buffer solubilization for each form also depended on the tissue used and in all cases decreased with rising environmental temperature. These two facts are taken to reflect interactions between acetylcholinesterase and membrane that depend on the nature of the membrane and probably involve the aliphatic chains of its phospholipids. The greater case of solubilization found in the low temperature adapted fish may be a function of the increased fluidity of the cellular membranes formed at lower temperatures.
对适应15摄氏度和25摄氏度的金鱼体内多种形式的乙酰胆碱酯酶进行了研究。通过蔗糖梯度离心法可区分出三种酶形式（沉降系数分别为18S、12S和5S）。它们的分子和酶学特性不随环境温度变化。值得注意的是，脑提取物和肌肉提取物中这三种形式的相对比例不同，18S形式在肌肉中占主导（约75%），12S形式在脑中更为丰富，这表明这些形式之间存在生理分化。在分别适应15摄氏度和25摄氏度的金鱼中，后一种形式分别从48%变化到70%。每种形式在盐缓冲液中溶解的相对效率也取决于所用组织，并且在所有情况下都随环境温度升高而降低。这两个事实被认为反映了乙酰胆碱酯酶与膜之间的相互作用，这种相互作用取决于膜的性质，可能涉及膜磷脂的脂肪链。在低温适应的鱼中发现的更高的溶解率可能是低温下形成的细胞膜流动性增加的结果。
