Vernoux J P, Bagnis R
Biochimie. 1976;58(4):479-84. doi: 10.1016/s0300-9084(76)80259-3.
A modification of the extraction procedure for obtaining ciguatoxic substances from the tissues of various poisonous reef fish has been developed. Rapid alkali treatment of the residue obtained at an early stage is efficient in separating the ciguatoxic fraction from toxic impurities which interfere with pharmacological tests. It is thus possibles to carry out a more precise dosage of the material separated in this way. Alkali treatment does not affect either the physiological activity in cats and mice of the ciguatoxic substances nor their behaviour in a preparative thin layer chromatography in a benzene-butanol system.
已开发出一种从各种有毒珊瑚礁鱼组织中获取雪卡毒素物质的提取程序的改进方法。对早期获得的残渣进行快速碱处理，可有效从干扰药理试验的有毒杂质中分离出雪卡毒素部分。因此，可以对以这种方式分离出的物质进行更精确的剂量测定。碱处理既不影响雪卡毒素物质在猫和小鼠中的生理活性，也不影响其在苯 - 丁醇系统中的制备薄层色谱行为。
