Shi X, Zhang Q
Department of Histology and Embryology, Henan Medical University, Zhengzhou.
Zhen Ci Yan Jiu. 1996;21(3):60-2.
Guinea pigs were acupunctured at "ZuSanli" (ST 36) with one direction twirling manipulation until an objective sensantion was felt by operator's hand. Frozen sections and scanning electromicroscopic specimens were made from the whole guinea leg acupunctured and were observed under light microscope and scanning electromicroscope respectively. In all specimens, a puncturing pore and different transformation of surrounding tissue were seen clearly. In hypodermis the connective tissue fibers circling the puncturing pore appeared as whirly form. In muscle layer, the connective tissue fibers of endomysium circled the pore and the relative muscle fiber were twisted and dislocated. Adjacent vessels and nerves were dislocated and transformed. It is infered that various needling sensative tissues and structures were simultaneously stimulated by twirling needle force, with connective tissue being as mediator, which is likely of the biological basis of needling sensation and ist complexity.
选用豚鼠，针刺“足三里”（ST 36），采用单向捻转手法，直至术者手下有针感。取针刺后的豚鼠全腿制作冰冻切片及扫描电镜标本，分别在光学显微镜和扫描电镜下观察。在所有标本中，均可清晰见到针刺针孔及周围组织的不同改变。皮下组织中，围绕针孔的结缔组织纤维呈漩涡状。肌层中，肌内膜的结缔组织纤维围绕针孔，相关肌纤维扭曲、移位。相邻血管和神经移位、变形。推测捻转针力同时刺激了多种针感组织和结构，结缔组织作为介导，这可能是针感及其复杂性的生物学基础。
