School of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
J Tradit Chin Med. 2019 Oct;39(5):730-739.
To analyze the effect of different twirling and rotating acupuncture manipulation techniques on the blood flow perfusion at acupoints to provide a reference for the study of acupoint specificity and the quantification and effectiveness of acupuncture methods.
Twenty healthy male or female college students each received four different acupuncture manipulation techniques, including simple acupuncture, uniform reinforcing-reducing needling, twirling reinforcing needling, and twisting reducing needling. The self-control method was applied. Acupuncture was performed by an acupuncture manipulation simulator at Neiguan (PC 6) and Zusanli (ST 36). The process of twirling and rotating was divided into seven timepoints. The PeriCam Perfusion Speckle Imager (PSI) System was used to collect the blood flow perfusion data at each acupoint. The specificity of twirling and rotating acupuncture manipulation was analyzed based on changes in the curve, video, and numerical blood flow perfusion data at each timepoint.
There were two peaks in the blood flow perfusion curve of twirling and rotating acupuncture manipulation; one appeared after 3 min of needle retention, and the other appeared 3 min after needle removal. The blood flow perfusion parameters showed that the greatest differences between the four manipulation techniques occurred after 5 and 10 min of needle retention. The specificity of various manipulation techniques was most obvious at these two timepoints. There were significant differences between the four manipulation groups in the blood flow perfusion and the relative change rates of blood flow perfusion at each timepoint.
Laser speckle imaging enables the real-time, non-invasive, rapid, and accurate collection of blood flow perfusion data during acupuncture. This imaging technique enables the easy attainment of various parameters such as visual images, two-dimensional curves, and data tables. At various timepoints, the four groups significantly differed regarding changes in blood flow perfusion and relative change rates of blood flow perfusion, which facilitated the differentiation of the four acupuncture manipulation methods. Based on this, further analysis could be conducted to study spatial distribution characteristics such as the influence area and flare area. The frequency domain analysis of acupuncture manipulation curves is important in the study of the dose-effect relationship and specificity of acupuncture manipulation.
分析不同捻转补泻针刺手法对穴位血流灌注的影响，为穴位特异性和针刺方法的量化及有效性研究提供参考。
20 名健康的男女大学生分别接受 4 种不同的针刺手法，包括单纯针刺、均匀提插针刺、捻转补法针刺和捻转泻法针刺。采用自身对照法，针刺操作模拟器针刺内关（PC6）和足三里（ST36）。捻转旋转过程分为 7 个时间点，应用 PeriCam 血流灌注斑点成像（PSI）系统采集各穴位血流灌注数据。根据各时间点血流灌注曲线、视频和数值的变化，分析捻转旋转针刺手法的特异性。
捻转旋转针刺手法的血流灌注曲线有 2 个峰值，一个出现在留针 3 min 后，另一个出现在出针 3 min 后。血流灌注参数显示，4 种手法在留针 5、10 min 时差异最大。在这两个时间点，各种手法的特异性最为明显。4 组在各时间点的血流灌注及血流灌注相对变化率均有显著差异。
激光散斑成像技术能够实时、无创、快速、准确地采集针刺过程中的血流灌注数据。这种成像技术可以轻松获得各种参数，如视觉图像、二维曲线和数据表。在各个时间点，4 组在血流灌注变化和血流灌注相对变化率方面均有显著差异，有利于区分 4 种针刺手法。在此基础上，可以进一步分析研究针刺手法的空间分布特征，如影响面积和扩散面积。针刺手法曲线的频域分析在针刺手法的量效关系和特异性研究中具有重要意义。
