Huang J, Yi Z, Lin G
Union Hospital, Fujian Medical College, Fuzhou.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1996;31(2):72-4.
The epidermoid formation (EF) in the developing human fetal middle ear is believed to have relationship with congenital cholesteatoma. Sixteen EFs were included in this study, of which 15 were simple EFs and one was hyperplastic EF. Four cytokeratin antibodies PK, CK13, CK18, and CK19 were used in an ABC immunohistochemical technique to compare the expression of the cytokeratin in the EFs, middle ear mucosa and external acoustic meatus skin. The results showed that the simple EFs had the same staining pattern of middle ear mucosa for all four antibodies and were different from external acoustic meatus skin. It was suggested that the simple EFs do not have the properties of an epidermis. The hyperplastic EF had an altered cytokeratin expression comparing to that of the middle ear mucosa. The expression of CK19 disappeared in the structure while the normal middle ear mucosa nearby had a positive staining for this antibody. PK staining pattern, characterized by the more intensive in the surface layer of the EF, was similar to that of external acoustic meatus skin. It is suggested that hyperplastic EF may acquire certain properties of epidermis, and may develop into congenital cholesteatoma.
在发育中的人类胎儿中耳中，表皮样形成（EF）被认为与先天性胆脂瘤有关。本研究纳入了16个EF，其中15个为单纯EF，1个为增生性EF。采用ABC免疫组织化学技术，使用四种细胞角蛋白抗体PK、CK13、CK18和CK19，比较EF、中耳黏膜和外耳道皮肤中细胞角蛋白的表达。结果显示，单纯EF对所有四种抗体的染色模式与中耳黏膜相同，与外耳道皮肤不同。提示单纯EF不具有表皮的特性。增生性EF与中耳黏膜相比，细胞角蛋白表达发生改变。CK19在该结构中的表达消失，而附近正常中耳黏膜对该抗体呈阳性染色。PK染色模式在EF表层更为强烈，与外耳道皮肤相似。提示增生性EF可能获得了表皮的某些特性，并可能发展为先天性胆脂瘤。
