Siebert R, Weber-Matthiesen K
Department of Human Genetics, University of Kiel, Germany.
Histochem Cell Biol. 1997 Oct-Nov;108(4-5):391-402. doi: 10.1007/s004180050180.
Primary and secondary chromosomal abnormalities play an important role in the characterization of biological, pathological, and clinical subgroups of malignant lymphomas. The introduction of fluorescence in situ hybridization (FISH) and the combination of immunophenotyping plus FISH to the diagnosis of lymphatic neoplasms allows the fast and sensitive detection of specific chromosomal changes and provides new insights into the genetic basis of lymphomagenesis. This article reviews the possibilities and limitations of molecular cytogenetic techniques in comparison to cytogenetic and molecular genetic methods and discusses their clinicopathological impact for non-Hodgkin's lymphoma and Hodgkin's disease.
原发性和继发性染色体异常在恶性淋巴瘤的生物学、病理学及临床亚组特征中发挥着重要作用。荧光原位杂交（FISH）技术的引入以及免疫表型分析与FISH相结合应用于淋巴瘤诊断，能够快速且灵敏地检测出特定的染色体变化，并为淋巴瘤发生的遗传基础提供新的见解。本文回顾了分子细胞遗传学技术相较于细胞遗传学和分子遗传学方法的可能性和局限性，并讨论了它们对非霍奇金淋巴瘤和霍奇金病的临床病理影响。
