Napierała M, Krzyzosiak W J
Laboratory of Cancer Genetics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
J Biol Chem. 1997 Dec 5;272(49):31079-85. doi: 10.1074/jbc.272.49.31079.
We show that CUG repeats form "slippery" hairpins in their natural sequence context of the myotonin kinase gene transcript. This novel type of RNA structure is characterized by strong S1 and T1 nuclease and lead cleavages in the terminal loop and by mild lead cleavages in the hairpin stem. The latter effect indicates a relaxed metastable structure of the stem. (CUG)5 repeats do not form any detectable secondary structure, whereas hairpins of increasing stability are formed by (CUG)11, (CUG)21, and (CUG)49. The potential role of the RNA hairpin structure in the pathogenesis of myotonic dystrophy is discussed.
我们发现，在肌强直性营养不良激酶基因转录本的天然序列背景中，CUG重复序列形成了“滑动”发夹结构。这种新型RNA结构的特征在于，在末端环中存在强烈的S1和T1核酸酶切割以及铅切割，而在发夹茎中存在轻度的铅切割。后一种效应表明茎具有松弛的亚稳态结构。(CUG)5重复序列不形成任何可检测到的二级结构，而(CUG)11、(CUG)21和(CUG)49则形成稳定性不断增加的发夹结构。本文讨论了RNA发夹结构在强直性肌营养不良发病机制中的潜在作用。
