Morishita F, Nakanishi Y, Kaku S, Furukawa Y, Ohta S, Hirata T, Ohtani M, Fujisawa Y, Muneoka Y, Matsushima O
Department of Biological Science, Faculty of Science, Hiroshima University, Japan.
Biochem Biophys Res Commun. 1997 Nov 17;240(2):354-8. doi: 10.1006/bbrc.1997.7659.
A novel cardio-excitatory peptide was purified from the hearts of Aplysia kurodai. The peptide was a tripeptide containing a D-amino acid residue in the second position, Asn-D-Trp-Phe-NH2 (NdWFamide). NdWFamide increased the amplitude of contractions of the perfused Aplysia heart with little effect on beating frequency, showing a threshold of approximately 10(-11) M. The peptide also potentiated spontaneous contractions of the anterior aorta. The synthetic peptide having L-Trp instead of D-Trp was about 1,000 times less potent than the native one. NdWFamide seems to play an important role in regulation of cardiac activity in Aplysia.
从黑指纹海兔的心脏中纯化出一种新型的心脏兴奋肽。该肽是一种三肽，在第二位含有一个D-氨基酸残基，即天冬酰胺-D-色氨酸-苯丙氨酸-酰胺（NdWFamide）。NdWFamide增加了灌注的黑指纹海兔心脏的收缩幅度，对搏动频率影响很小，其阈值约为10^(-11) M。该肽还增强了前主动脉的自发收缩。用L-色氨酸代替D-色氨酸的合成肽的效力比天然肽低约1000倍。NdWFamide似乎在黑指纹海兔心脏活动的调节中起重要作用。
